Erythropoietin concentrated solution. Determination of isoforms according to Ph. Eur. 1316

INTRODUCTION

CAPEL capillary electrophoresis system is a versatile analytical tool for pharmaceutical analysis of proteins, peptides and small molecules using capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF) and micellar electrokinetic chromatography (MEKC). CAPEL is suitable for the analysis by capillary electrophoresis according to any monograph or general chapter from world pharmacopoeias.

The present method is used for the determination of erythropoietin (EPO) isoforms in erythropoietin concentrated solutions according to European Pharmacopoeia monograph 1316 (Identification, method B. Capillary zone electrophoresis) using CAPEL capillary electrophoresis system.