Determination of synthetic amino acids (methionine, threonine, tryptophan) in fodder additives
INTRODUCTION
The method is used for the determination of the mass fraction of free methionine, threonine, and tryptophan in fodder additives.
For the determination of the mass fraction of methionine, threonine, and tryptophan in feedstuffs, compound feeds, fodders, premixes, and all types of feed raw materials use the method M 04-38-2009 (Lumex Instruments kit, order No 0300002027).
For the determination of free lysine in fodder additives use the method М 04-87-2016 (Lumex Instruments set, order No 0300002726).
For the determination of methionine hydroxy analogue (HMTBa) in fodder additives use the method М 04-83-2014 (Lumex Instruments set, order No 0300001888).
MEASURING METHOD
The measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 200 nm.