Ready-to-run microchips for identification of African swine fever

African swine fever virus (ASFV), a large double-stranded DNA agent causes complex, hemorrhagic, and contagious disease in domestic and wild swine leading to significant socio-economic impact. In the early diagnosis of ASFV, passive surveillance involving clinical signs and fatality data of swine is considered pivotal. However, for a rapid and early diagnosis, the surveillance must have adequate laboratory support in detection & identification of AFSV. Recommended tests for virus detection include enzyme-linked immunosorbent assay (ELISA), and PCR assays. Although ELISA allows large-scale testing of the samples, it has been reported as having lower analytical sensitivity than conventional or real-time PCR tests.

To improve the desired rapidity, cost-effectiveness, sensitivity, and specificity of the detection process, Lumex is offering ready-to-run microchip kit with real-time PCR analyzer AriaDNA. This technology has ability to run pathogen testing both in the laboratory and on swine farms. In addition, for reliable results, a set of positive & negative controls for each of the pathogen, internal control (IC) as an internal inhibition monitor that is amplified in parallel with the target DNA are also included in the microchip.